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ERX12122385: RNAseq of submergence response of the flood tolerant Cardamineae tribe
9 ILLUMINA (Illumina HiSeq 4000) runs: 49.4M spots, 9.9G bases, 3.7Gb downloads

Design: RNAseq of submergence response of the flood tolerant Cardamineae tribe
Submitted by: EBI (European Bioinformatics Institute)
Study: RNAseq of submergence response of the flood tolerant Cardamineae tribe
show Abstracthide Abstract
Pot grown plants of Arabidopsis thaliana, Cardamine hirsuta, Cardamine pratensis, Rorippa palustris and Rorippa sylvestris where completely submerged under ambient light conditions. After 24 and 48 hours the shoots were harvested for expression analysis. Differential expression analysis, taking into account unsubmerged control plants revealed that the Rorippa genus had a pronounced down regulation of the cell cycle whereas the Cardamine had an attenuated response to submergence.
Sample: Rpal_subm1_II
SAMEA115417750 • ERS18444762 • All experiments • All runs
Library:
Name: Rpal_subm1_II_p
Instrument: Illumina HiSeq 4000
Strategy: ssRNA-seq
Source: TRANSCRIPTOMIC
Selection: RT-PCR
Layout: PAIRED
Construction protocol: young leaves including shoot apical meristems were harvested and immediately snap frozen in liquid nitrogen All plant species were grown on a soil mixture until the 10-leaf-stage, about 3-4 weeks after germination, according to established protocols (Müller et al. 2019), under short-day conditions (8 h light, 16 h darkness), 100 µmol photons * m-2 * s-1 and 23 °C. Submergence stress was applied to the plants by immersing them in big, transparent boxes (about 50 l volume) filled with 40 l of tap water 24 h before start of the treatment. Treatment was started 2 hours after start of illumination. Frozen samples were ground for 3 min in 2 ml tubes with zirconia/silica beads using a Mini-Beadbeater (BioSpec Products, Bartlesville, OK, USA) in plates frozen with liquid nitrogen. After grinding, 1 ml lysis binding buffer (Dynabeads mRNA Direct Kit; Thermo Fisher Scientific, Waltham, MA, USA) was added and samples were homogenized with this buffer for an additional 2 min in the Beadbeater. Homogenized samples were centrifuged at 12 000 g for 3 min and loaded onto a homogenizer spin column (Omega Bio-Tek, Norcross, GA, USA) and centrifuged to remove excess tissue. Lysates were transferred to a 96-well plate and stored at −80°C until library preparations. RNA-seq libraries were constructed using breath adapter directional sequencing (BrAD-seq) protocol for non-strand-specific libraries (Townsley et al., 2015). Briefly, 200 μl of lysates was used for messenger RNA (mRNA) isolation, following the manufacturer's instructions (Dynabeads mRNA Direct Kit; Thermo Fisher Scientific). Isolated mRNA was fragmented by reverse transcription (RT) buffer for 90 s at 94°C (RevertAid RT enzyme; Thermo Fisher Scientific), and complementary DNA (cDNA) synthesis was done on the fragmented mRNA. Second-strand synthesis was done according to BrAD-seq protocol and was followed by adapter ligation. PCR enrichment was performed using unique indexed oligos for multiplexing.
Runs: 9 runs, 49.4M spots, 9.9G bases, 3.7Gb
Run# of Spots# of BasesSizePublished
ERR127490285,833,9611.2G449.1Mb2024-07-23
ERR127490275,835,8261.2G449.6Mb2024-07-23
ERR127490235,783,7271.2G444.4Mb2024-07-23
ERR127490215,821,0661.2G444.3Mb2024-07-23
ERR127490245,825,4041.2G450.8Mb2024-07-23
ERR127490254,351,627872.6M330Mb2024-07-23
ERR127490204,520,031906.6M360.5Mb2024-07-23
ERR127490225,792,7631.2G445.7Mb2024-07-24
ERR127490265,671,0801.1G434.5Mb2024-07-24

ID:
34052761

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